This monoclonal antibody provides highly specific detection of the DYKDDDDK (FLAG) epitope tag, widely used in recombinant protein expression and purification. Clone 1D2 delivers strong affinity and low background in ELISA, Western blotting, immunoprecipitation, and protein‑characterization workflows. Its clean, reproducible signal enables precise monitoring of FLAG‑tagged proteins in cell lysates, culture supernatants, and purified preparations. MFLG‑45A is ideal for molecular biology, protein engineering, and assay development requiring reliable detection of FLAG‑tagged constructs.